MagPlex^® Monitoring Microspheres

Monitor assay and instrument performance

MagPlex^® Monitoring Microspheres are magnetic polystyrene beads that provide insight into factors impacting target median fluorescent intensity (MFI) values, including instrument fluctuations and user error.

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MagPlex® Monitoring Microspheres for Luminex instruments

Features

Magnetic Microspheres. MagPlex^® Monitoring Microspheres are magnetic polystyrene microparticles that have been color coded to classify to a specific region.
Additional reporter dye. In addition to the classification dyes, MagPlex^® Monitoring Microsphere contain an internal reporter dye that generates reporter median fluorescent intensity (MFI) values specific to instrument type and reporter modes.
Microsphere interrogation. The MagPlex^® Monitoring Microsphere is excited by the optical system of the xMAP^® Instrument, and resulting MFIs can be analyzed for indications of performance errors.

Benefits

Increased confidence in your results: Monitor well-to-well assay performance for errors, ensuring each individual well has not been impacted by external factors.
Broad application areas: These microspheres can be incorporated into various assay formats, including proteomic and genomic applications.
The ability to pinpoint errors quickly: MFI values generated using these microspheres can eliminate instrument and user errors as a source of unexpected results.

Workflow

MagPlex^® Monitoring Microspheres can be incorporated into new or existing assays.

Incorporate Magplex® Monitor Beads Into The Assay Bead Mix - Diasorin

Incorporate MagPlex^® Monitoring Microspheres into bead mix

After assay validation, incorporate MagPlex^® Monitor beads into the assay bead mix.
Perform assay steps as normal.

Analyze samples

Analyze each sample well, containing both the assay and monitoring beads, on an xMAP^® instrument.

Review results

Compare results to validated assay thresholds to verify there were no user errors or instrument malfunctions.

Performance

	MagPlex^® RP1 Monitor	MagPlex^® RP2 Monitor
Magnetic	Yes	Yes
Assigned Region	045	222
RP1 MFI Value Range	MAGPIX^® 2,000-3,000 Luminex^®200^™ Default PMT: 2,000-3,000 High PMT: 9,100-13,650 FLEXMAP 3D^® Standard PMT: 2,000-3,000 High PMT: 9,100-13,650 ×MAP INTELLIFLEX^® Systems Luminex^® 200^™ Mode Low PMT: 2,000-3,000 High PMT: 9,100-13,650 FLEXMAP 3D^® Mode Low PMT• 3,400-5,100 High PMT: 15,470-23,205 xMAP INTELLIFLEX^® Mode Dual Reporter: 2,000-3,000 High Sensitivity: 260,000-390,000	N/A
RP2 MFI Value Range	N/A	×MAP INTELLIFLEX^® DR-SE Dual Reporter: 2,000-3,000
Instrument compatibility	MAGPIX^® Luminex^® 200^™ FLEXMAP 3D^® xMAP INTELLIFLEX^® xMAP INTELLIFLEX^® DR-SE	xMAP INTELLIFLEX^® DR-SE

To be used on

MagPlex^® Monitoring Microspheres are compatible with all xMAP^® Instruments.

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