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MagPlex® Monitoring Microspheres

Monitor assay and instrument performance

MagPlex® Monitoring Microspheres are magnetic polystyrene beads that provide insight into factors impacting target median fluorescent intensity (MFI) values, including instrument fluctuations and user error.

MagPlex® Monitoring Microspheres for Luminex instruments

Features

  • Magnetic Microspheres. MagPlex® Monitoring Microspheres are magnetic polystyrene microparticles that have been color coded to classify to a specific region.
  • Additional reporter dye. In addition to the classification dyes, MagPlex® Monitoring Microsphere contain an internal reporter dye that generates reporter median fluorescent intensity (MFI) values specific to instrument type and reporter modes.
  • Microsphere interrogation. The MagPlex® Monitoring Microsphere is excited by the optical system of the xMAP® Instrument, and resulting MFIs can be analyzed for indications of performance errors. 

Benefits

  • Increased confidence in your results: Monitor well-to-well assay performance for errors, ensuring each individual well has not been impacted by external factors.
  • Broad application areas: These microspheres can be incorporated into various assay formats, including proteomic and genomic applications.
  • The ability to pinpoint errors quickly: MFI values generated using these microspheres can eliminate instrument and user errors as a source of unexpected results.

Workflow

MagPlex® Monitoring Microspheres can be incorporated into new or existing assays.

Incorporate Magplex® Monitor Beads Into The Assay Bead Mix - Diasorin
Incorporate MagPlex® Monitoring Microspheres into bead mix

After assay validation, incorporate MagPlex® Monitor beads into the assay bead mix.
Perform assay steps as normal.

Incorporate Magplex® Monitor Beads Into The Assay Bead Mix - Diasorin
Analyze samples

Analyze each sample well, containing both the assay and monitoring beads, on an xMAP® instrument.

Incorporate Magplex® Monitor Beads Into The Assay Bead Mix - Diasorin
Review results

Compare results to validated assay thresholds to verify there were no user errors or instrument malfunctions.

Performance

MagPlex® RP1 Monitor
MagPlex® RP2 Monitor
Magnetic

Yes

Yes

Assigned Region

045

222

RP1 MFI Value Range

MAGPIX®
2,000-3,000

Luminex® 200
Default PMT: 2,000-3,000
High PMT: 9,100-13,650

FLEXMAP 3D® 
Standard PMT: 2,000-3,000
High PMT: 9,100-13,650

×MAP INTELLIFLEX® Systems
Luminex® 200 Mode

Low PMT: 2,000-3,000
High PMT: 9,100-13,650

FLEXMAP 3D® Mode
Low PMT• 3,400-5,100
High PMT: 15,470-23,205

xMAP INTELLIFLEX® Mode
Dual Reporter: 2,000-3,000
High Sensitivity: 260,000-390,000

N/A

RP2 MFI Value Range

N/A

×MAP INTELLIFLEX® DR-SE
Dual Reporter: 2,000-3,000

Instrument compatibility

MAGPIX®
Luminex® 200
FLEXMAP 3D®
xMAP INTELLIFLEX®
xMAP INTELLIFLEX® DR-SE

xMAP INTELLIFLEX® DR-SE

To be used on

 MagPlex® Monitoring Microspheres are compatible with all xMAP® Instruments.

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MagPlex® Monitoring Microspheres ordering info

Product name
Volume
Concentration (microspheres/mL)
Region
Registration status
Part number
MagPlex® RP1 Monitor

1 mL

1.25 x 10

45

For Further Manufacture

MRP1-045-01

MagPlex® RP2 Monitor

1 mL

1.25 x 10

222

For Further Manufacture

MRP2-222-01

Resources

xMAP Insights magazine

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xMAP Resource Round-Up

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Research publications

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Document repository

Product documentation

White papers

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xMAP® Kit Finder

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Partners

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More details about MagPlex® Monitoring Microspheres

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Delivering the versatility to perform a wide range of protein and nucleic acid-based multiplex assays—on a single platform.

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