xMAP® Technology is commonly used for:
- Biomarker characterization
- Identification of secreted proteins
- Mechanism of action studies
- Patient stratification
- Therapeutic drug monitoring
- Gene expression analysis
- Single nucleotide polymorphism (SNP) detection
- Pathogen detection and typing
- Exosome studies
- MicroRNA analysis
xMAP® in action
Explore xMAP® Technology and discover some of the many applications used
by multiplexing research experts across the globe.
xMAP® Assay Automation
Automation can boost productivity, minimize errors, and save on reagents.
Bead-Based Multiplexing vs. Electrochemiluminescence
Bead-based multiplexing offers significantly higher plex capacity, requires less hands-on time and delivers superior results compared to traditional methods.
For immunoassays, the capture sandwich format is commonly used for xMAP® protein assays, where the analyte is “sandwiched” between a capture and detection antibody. The capture sandwich format is compatible with a wide variety of target analytes.
A competitive format is used when the target analyte has a low molecular weight and only one or two antibody-binding epitopes or if only one antibody is available. Competitive xMAP® assays can be developed using either antibody-coupled or antigen-coupled beads and can be multiplexed with the capture sandwich format in the same assay.
An indirect format is used for serological assays to measure the amount of antigen-specific antibody present in biological samples. Once a format has been chosen, the beads can be coupled to the proteins used in the assay.
Nucleic Acid Assays
The open architecture of xMAP® Technology enables numerous nucleic acid assay applications. Multiplex nucleic acid assays are developed by coupling user-defined sequence-specific capture oligos to MagPlex® beads or using MagPlex®-TAG™ beads that are already coupled to unique 24-base DNA sequences (anti-TAGs).
What can be coupled to beads
Looking for custom assay design and development services with unique targets in mind? Discover the LuminexPLORE Lab and partner with the xMAP® multiplexing industry experts.
xMAP® Kit Finder
xMAP® Technology is an open multiplexing platform used in hundreds of commercial immunoassay kits. Industry leading manufacturers offer xMAP® Technology assay kits. To find them, use our xMAP® Kit Finder.