MagPlex^® Monitoring Microspheres

Monitor assay and instrument performance

About

MagPlex^® Monitoring Microspheres are magnetic polystyrene beads that provide insight into factors impacting target median fluorescent intensity (MFI) values, including instrument fluctuations and user error.

All microspheres are general-use components for further manufacture of xMAP^® bioassays.

Features

Magnetic Microspheres: MagPlex^® Monitoring Microspheres are magnetic polystyrene microparticles that have been color-coded to classify to a specific region.
Additional reporter dye: In addition to the classification dyes, MagPlex^® Monitoring Microspheres contain an internal reporter dye that generates reporter median fluorescent intensity (MFI) values specific to instrument-type and reporter modes.
Microsphere interrogation: The MagPlex^® Monitoring Microsphere is excited by the optical system of the xMAP^® Instrument, and resulting MFIs can be analyzed for indications of performance errors.

Benefits

Increased confidence in your results: Monitor well-to-well assay performance for errors, ensuring each individual well has not been impacted by external factors.
Broad application areas: These microspheres can be incorporated into various assay formats, including proteomic and genomic applications.
The ability to pinpoint errors quickly: MFI values generated using these microspheres can eliminate instrument and user errors as a source of unexpected results.

Workflow

MagPlex^® Monitoring Microspheres can be incorporated into new or existing assays.

Incorporate Magplex® Monitor Beads Into The Assay Bead Mix - Diasorin

Incorporate MagPlex^® Monitoring Microspheres into bead mix

After assay validation, incorporate MagPlex^® Monitor beads into the assay bead mix.
Perform assay steps as normal.

Analyze samples

Analyze each sample well, containing both the assay and monitoring beads, on an xMAP^® instrument.

Review results

Compare results to validated assay thresholds to verify there are no user errors or instrument malfunctions.

Performance

	MagPlex^® RP1 Monitor	MagPlex^® RP2 Monitor
Magnetic	Yes	Yes
Assigned Region	045	222
RP1 MFI Value Range	MAGPIX^® 2,000-3,000 Luminex^®200^™ Default PMT: 2,000-3,000 High PMT: 9,100-13,650 FLEXMAP 3D^® Standard PMT: 2,000-3,000 High PMT: 9,100-13,650 xMAP INTELLIFLEX^® Systems Luminex^® 200^™ Mode Low PMT: 2,000-3,000 High PMT: 9,100-13,650 FLEXMAP 3D^® Mode Low PMT• 3,400-5,100 High PMT: 15,470-23,205 xMAP INTELLIFLEX^® Mode Dual Reporter: 2,000-3,000 High Sensitivity: 260,000-390,000	N/A
RP2 MFI Value Range	N/A	xMAP INTELLIFLEX^® DR-SE Dual Reporter: 2,000-3,000
Instrument Compatibility	MAGPIX^® Luminex^® 200^™ FLEXMAP 3D^® xMAP INTELLIFLEX^® xMAP INTELLIFLEX^® DR-SE*	xMAP INTELLIFLEX^® DR-SE*
*For research use only. Not for use in diagnostic procedures.

To be used on

Multiplexing systems designed for ease of use, flexibility, and scalability to enable custom assay development for protein and nucleic acid-based multiplex testing.